Flow cytometry is an indispensable tool in clinical and basic research for the diagnosis, monitoring and follow-up of disease progression.
It aims to provide a reliable method for the rapid and precise analysis of cellular and other particulate suspensions. The main goals of the technology are the examination of cells from peripheral blood and to provide a useful tool for the investigation of cellular characteristics, cellular functions and cellular abnormalities. It is also well suited for qualitative and/or quantitative readout of predefined parameters optimised for microbeads. It can also be used for DNA analysis by measuring the DNA content of cells. For example, by labelling the DNA content of cells with chromophore molecules, abnormal DNA content can be detected.
Fluorescence Activated Cell Sorting (FACS) is a method for counting, sorting and isolating particles (e.g. living cells).
Flow cytometers equipped with a cell sorter can be used to easily and efficiently isolate selected populations from any cell or particle mixture, allowing their further analysis. For live cell samples, it is also possible to further culture the selected cells.